Morphometric study of biceps braquial muscle fibers of rats submited a swimming exercise associated with the comjugate use of the two anabolic steroids hormone.

Abstract

The use of anabolic steroids increases mainly: the power, acceleration and muscle explosion. The research aimed to quantify and compare the development in the two types of muscle fibers (type I and type II) of the brachial biceps muscle in sedentary and trained rats, underwent a supratherapeutic dose of two simultaneous anabolic steroids. To this end, we used 40 rats were divided into four groups, namely: sedentary, sedentary anabolic , trained and trained anabolic. The training was swimming, 1 hour per day, 6 times a week for 9 weeks. The anabolic steroids used were the stanozolol (50 mg / ml) and of nandrolone decanoate (50 mg / ml). The therapeutic dose equivalent proportion to the dosage indicated on the package leaflet for an adult of 70 kg. After the period of training the rats were sacrificed and brachial biceps muscle collected, criofixado in liquid nitrogen, cut into cryostat and stained with HE and the reaction to histochemical NADH-TR. The extent of the cross section area of muscle, the three experimental groups, showed no statistical difference when compared to sedentary control group. The fibers showed that small size and intense dark blue color were the Type I and ranged from 399 µm2 to 2427 μm2, which showed the size and color, were the clear blue Type IIb, and the size varied from 943 µm2 to 5787µm2. Another contingent of fibers that had size and coloring agent were type IIa μm2 ranging from 705 to 4351 µm2. We conclude that the reaction of muscle to exercise and the use of a hormone anabolizing, even at supra-physiological dose, was nil, since there was no increase in the size of the fibers as was expected. The adaptation of muscle to the experimental protocol was not evidenced by the increase in the size of fiber, but by biochemical changes that do not translate by the hypertrophy, as the type of exercise performed is not the most conducive to such stimuli.