Molecular mechanisms on induction obesity and insulin resistance in detrained animals submitted a high-fat diet

Authors

  • José Rodrigo Pauli Laboratório de investigação dos mecanismos moleculares de sinalização da insulina, FCM/Unicamp-Campinas, SP
  • Dennys Esper Cintra Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp SP
  • Eduardo Rochete Ropelle Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp SP
  • Cláudio Teodoro de Souza Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP
  • Juliana Contin Moraes Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP
  • Mário José Abdalla Saad Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP

DOI:

https://doi.org/10.5016/814

Keywords:

Destreinamento. Obesidade. Resistência à insulina.

Abstract

The termination of exercise training (detraining) results in rapid fat accretion, weight gain and insulin resistance in both humans and rats. There is evident relationship between physical inactivity and insulin resistance. Different mechanisms may be involved in insulin resistance in this animal model. The aim of this study was to investigate the PI3-kinase/Akt and CAP/Cbl signaling pathways both involved with insulin-stimulated glucose uptake in white adipose tissue in detrained and sedentary animals submitted a high-fat diet. Wistar rats were submitted to swimming training during 8 weeks. Next this period the animals stop the training and received a rich-fat diet. The proteins from the insulin signaling pathway were analyzed by immunoprecipitation and immunoblotting. The results demonstrated that detraining result in an increased body mass and rapid body fat accretion. This fact was associated with increases insulin responsiveness in adipose tissue through IRS/PI3-Kinase/Akt pathway in detrained rats fed with a rich-fat diet. In addition, the CAP/Cbl pathway in adipose tissue was more insulin responsives in the detrained animals feeding with a rich-fat diet than S-DHL animals. In conclusion, the cessation of exercise is accompanied by increased body mass and rapid fat accretion and this aspect to have relation at least in part to increased insulin responsiveness in adipose tissue through CAP/Cbl pathway.

Author Biographies

José Rodrigo Pauli, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina, FCM/Unicamp-Campinas, SP

Laboratório de investigação dos mecanismos moleculares de sinalização da insulina, FCM/Unicamp Campinas, SP, Brasil. http://lattes.cnpq.br/4940811853835506

Dennys Esper Cintra, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp SP

Doutorando em Clínica Médica pela Universidade Estadual de Campinas, SP, Brasil. http://lattes.cnpq.br/2329875440704117

Eduardo Rochete Ropelle, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp SP

Atua como professor assistente na disciplina de Iniciação a Pesquisa Científica I e II, no curso de medicina na Faculdade de Ciências Médicas da UNICAMP, Campinas, SP, Brasil. http://lattes.cnpq.br/1228052025477313

Cláudio Teodoro de Souza, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP

Atualmente é Pesquisador colaborador pela Unicamp, Campinas, SP, Brasil e orientador credenciado pela Unicsul. http://lattes.cnpq.br/0968990271115488

Juliana Contin Moraes, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP

Atualmente é doutoranda em Clínica Médica na Unicamp, bolsista FAPESP e pesquisa o efeito pró-apoptótico da dieta hiperlipídica nos neurônios hipotalâmicos de ratos obesos. http://lattes.cnpq.br/5902203567566406

Mário José Abdalla Saad, Laboratório de investigação dos mecanismos moleculares de sinalização da insulina do Departamento de Clínica Médica – FCM/Unicamp, Campinas, SP

Universidade Estadual de Campinas, Faculdade de Ciências Médicas, Departamento de Clínica Médica. Campinas, SP, Brasil http://lattes.cnpq.br/7159637189296890

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Published

2007-12-20

Issue

V. 13, N. 2 Suplemento (2007)

Section

Invited Paper and Award Paper

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